X-ray structure analysis of a membrane protein complex. Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center from Rhodopseudomonas viridis

J. Deisenhofer, O. Epp, K. Miki, R. Huber, H. Michel

Research output: Contribution to journalLetter

1475 Scopus citations

Abstract

X-ray analysis of three-dimensional crystals of the photosynthetic reaction center from the purple bacterium Rhodopseudomonas viridis led to an electron density distribution at 3 Å resolution calculated with phases from multiple isomorphous replacement. The protein subunits of the complex were identified. An atomic model of the prosthetic groups of the reaction center complex (4 bacteriochlorophyll b, 2 bacteriopheophytin b, 1 non-heme iron, 1 menaquinone, 4 heme groups) was built. The arrangement of the ring systems of the bacteriochlorophyll b and bacteriopheophytin b molecules shows a local 2-fold rotation symmetry; two bacteriochlorophyll b form a closely associated, non-covalently linked dimer ("special pair"). A different local 2-fold symmetry axis is observed for the heme groups of the cytochrome part.

Original languageEnglish (US)
Pages (from-to)385-398
Number of pages14
JournalJournal of Molecular Biology
Volume180
Issue number2
DOIs
StatePublished - Dec 5 1984

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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