Yeast artificial chromosome contigs reveal that distal variable-region genes reside at least 3 megabases from the joining regions in the murine immunoglobulin κ locus

Julia B. George, Shuyu Li, William T. Garrard

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The immunoglobulin κ gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (v(κ)- region genes generally considered to comprise 20 families. To delineate the locus we have isolated 29 yeast artificial chromosome genomic clones that form two contigs, span >3.5 megabases, and contain two known non- immunoglobulin κ markers. Using PCR primers specific for 19 V(κ) gene families and Southern analysis, we have refined the genetically defined order of these V(κ) gene families. Of these, V(κ)2 maps at least 3.0 Mb from the joining (J(κ)) region and appears to be the most distal V(κ) gene segment.

Original languageEnglish (US)
Pages (from-to)12421-12425
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number26
DOIs
StatePublished - Dec 19 1995

Keywords

  • PCR screening
  • genomic walking
  • physical mapping

ASJC Scopus subject areas

  • General

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