Abstract
The immunoglobulin κ gene locus encodes 95% of the light chains of murine antibody molecules and is thought to contain up to 300 variable (v(κ)- region genes generally considered to comprise 20 families. To delineate the locus we have isolated 29 yeast artificial chromosome genomic clones that form two contigs, span >3.5 megabases, and contain two known non- immunoglobulin κ markers. Using PCR primers specific for 19 V(κ) gene families and Southern analysis, we have refined the genetically defined order of these V(κ) gene families. Of these, V(κ)2 maps at least 3.0 Mb from the joining (J(κ)) region and appears to be the most distal V(κ) gene segment.
Original language | English (US) |
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Pages (from-to) | 12421-12425 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 92 |
Issue number | 26 |
DOIs | |
State | Published - Dec 19 1995 |
Keywords
- PCR screening
- genomic walking
- physical mapping
ASJC Scopus subject areas
- General