Yeast autonomously replicating sequence binding factor is involved in nucleotide excision repair

Simon H. Reed, Masahiro Akiyama, Bruce Stillman, Errol C. Friedberg

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Nucleotide excision repair (NER) in yeast is effected by the concerted action of a large complex of proteins. Recently, we identified a stable subcomplex containing the yeast Rad7 and Rad16 proteins. Here, we report the identification of autonomously replicating sequence binding factor 1 (ABF1) as a component of the Rad7/Rad16 NER subcomplex. Yeast ABF1 protein is encoded by an essential gene required for DNA replication, transcriptional regulation, and gene silencing. We show that ABF1 plays a direct role in NER in vitro. Additionally, consistent with a role of ABF1 protein in NER in vivo, we show that certain temperature-sensitive abf1 mutant strains that are defective in DNA replication are specifically defective in the removal of photoproducts by NER and are sensitive to killing by ultraviolet (UV) radiation. These studies define a novel and unexpected role for ABF1 protein during NER in yeast.

Original languageEnglish (US)
Pages (from-to)3052-3058
Number of pages7
JournalGenes and Development
Volume13
Issue number23
DOIs
StatePublished - Dec 1 1999

Keywords

  • ABF1
  • DNA repair
  • Rad16
  • Rad7
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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